Crest LFOV Spinning Disk/ C2 Confocal

FPBASE

Optics

Objectives

1. CFI Plan Apo Lambda 10x /0.45

2. CFI Plan Apo Lambda 20x/0.75

3. CFI Plan Apo Lambda 40x/0.95 Corr
4. CFI Plan Apo Lambda S 40x/1.25 Corr (Silicone)

5. CFI Plan Apo Lambda 60x/1.4

Filter Turret 1

1. DAPI

2. GFP

3. Texas Red

4. Empty

5. Empty

6. 405nm Reflect/430-800nm Trans Dual TIRF (FRAP)

Crest Dichroics

1. 405/488/561/640/750

2. 440/540

Emission Wheel (Crest)

1. Nikon Multiband

2. FF01-438/24
3. FF01-485/20
4. FF01-511/20

5. FF01-595/31

6. FF01-560/25
7. FF02-685/40
8. FF01-819/44

Hardware

• Nikon Ti2-E Microscope

• Crest X-Light V2 L-FOV spinning disk confocal

• Nikon C2 confocal with DUVb detector
• MCL piezo stage
• Opti-Microscan FRAP with 405nm laser line
• Lumencor Sola SE U-NIR
• Lumencor Celeste Light engine
• Prime 95B 25mm sCMOS camera

• Okolab enclosure and Bold Line incubator

Lasers

C2 Confocal

• 405 nm,

• 488 nm,

• 561 nm,

• 640 nm,

Crest Confocal (Lumencor Celeste Light engine)

• 405 nm

• 446 nm
• 477 nm
• 520 nm
• 546 nm
• 638 nm
• 749 nm

Interface Requirements

Firmware

PC

• Windows 10 Pro 64-bit

• Core i7 3.4GHz

• 16 GB RAM

• NVidia GTX550Ti

• 240GB SSD

• 2TB Hard drive (RAID 1)

Software

• Nikon Elements 5.41.01 build 1709

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