TIRF/N-STORM

FPBASE

Optics

Objectives

1. Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel

2. Plan Apo 20x/0.75 (DIC N2 / 20X) 785 nm/pixel

3. Empty

4. Plan Apo VC 60XA/1.20 Water

5. Plan Apo lambda 100x/1.45 TIRF Oil 157 nm/pixel

6. Apo TIRF 100x/1.49 Oil (DIC N2 / 100X I) 157 nm/pixel

Filter Turret 1 (FL1)

1. Empty

2. Analyzer

3. Quad N-STORM

4. DAPI/FITC/Rhodamine Triple (for eyepiece viewing with arc lamp)

5. Empty

6. Empty

Emission Wheel

1. ET455/50m

2. ET525/50m

3. ET600/60m

4. ET705/72m

5. ZET488/561/640m (triple bandpass)

6. DualBP 59012m (515/30m, 598/45m))

7. ZET405/488m-TRF

8. Empty

9. Blocker

10. Blocker

Hardware

• Nikon Ti-E Microscope

• Sutter emission wheel and 10-B (COM 5 baud rate auto) 

• Nikon motorized stage 

• Andor DU897 on right port with STORM lens

• Hammamatsu Orca Flash 4 (left port, COM 4). Currently has Dual view splitter with DV2-Cube (T5101pxrt, ET460/36m, Et545/40m)

• Agilent MLC400 (405nm, 488nm, 561nm, 647nm) with NIDAQ interface (v18.oo)

• Brightfield shutter controlled by Sutter Lambda SC through Ti

Interface Requirements

• Camera link board- Orca Flash 4 PCIe- 1x-CLD-2PE8 

• CCI-24 PCIe controller card- Andor DU897
• NI PCI 6723 board connected though PXI box -Agilent Lasers MLC 400B 
• R332 Serial connector- Intensilight 
• USB for Sutter 10-B 
• USB Oko lab bold line 
• USB - Nikon Ti

• USB MCL nano drive

Firmware

• Ti: Combination I

• TiControl: 5.2

PC

• Intel(R) Xeon(R) W-2245 CPU @ 3.90GHz, 3912 Mhz, 8 Core(s)

• 64 GB RAM

• 64 bit Windows 10 Pro

• NVidia RTX 4000

• 500GB C: Hard drive

• 1TB D: Hard drive (RAID 1)

Software

• NIS-Elements 5.20.00 build 1423