Weill- Stellaris 8 Tau STED

This is a super-resolution laser-scanning confocal outfitted with 3 STED depletion lasers. It is capable of standard laser-scanning confocal microscopy, STED, tau-STED, and FLIM. With the White-Light Laser (WLL) it can be used to image a wide variety of fluorophores and is capable of separating fluorophores spectrally close.

Optics

Objectives Installed

1. -
2. HC PL APO 86x/1.20 W motCORR STED W (Calibrated for Pos 2, occupies 2 positions)
3. HC PL APO 10x/0.4 NA CS2
4.  HC PL APO 20x/0.75 NA CS2
5.  HC PL APO 40x/1.30 Oil CS2
6. HC PL APO 100x/1.40 OIL STED W (Calibrated for Pos 6)

Objectives Stored off the microscope

1. HC PL APO 63x/1.40 OIL CS2 (Move to SD)
2. HC FLUOTAR L 25x/0.95 W VISIR
3. HC PL APO 93x/1.30 GLYC motC STED W (Calibrated for Pos 4, occupies 2 positions)
4. HC PL APO 40x/0.95 CORR

Hardware

• Leica DMi8 Inverted Microscope
• Leica Adaptive Focus Control (AFC)
• Scanning stage SCAN plus inverted motorized stage
• Z-Piezo stage with 500um travel (WieneckeSinske WSBPiezo)
• Water Immersion micro dispenser
• 8kHz Tandem Scanner STELLARIS 8 (galvo and resonant)
• HyD detectors
  • HyD S (pos 1 and pos 3)
  • HyD X (pos 2 and pos 4)
  • HyD R (pos 5)

Light Source

• Leica LED 3 (390-680nm) with 3mm LLG

Excitation Filters

• Widefield
  • LED 405 filter cube
  • CFP/YFP filter cube
  • GFP filter cube (not installed?, doesn’t show up in list on scope)
  • Rhod LP filter cube
  • DA/FI/TX filter cube

Lasers

• 405nm
• White Light Laser  (pulsed, 440 to 790 nm)
• 592 nm STED depletion laser
• 660 nm STED depletion laser
• 775 nm STED depletion laser

Laser Notch filters:

Fluorifier Disc #1:

• Empty
• NF 448/514/561
• NF 488/561/730
• NF 448/514
• NF 488/561/638

Fluorifier Disc #2:

• Empty
• NF 685
• NF 638
• NF 488/638
• NF 775
• NF 660

Detector

Interface Requirements

Firmware

PC

Software