FP BASE
This a super-resolution spinning disk confocal that can provide a 1.5 fold improvement in resolution over a standard spinning disk confocal. It is capable of imaging at video-rate speeds with a Photometrics Kinetex camera and is equipped with a Mightex DMD and a CO2 and temperature-controlled incubator for live-cell imaging.
Objectives:
- HC PL FL 10x/0.30
- HC PL APO 20x/0.80
- HC PL APO 40x/0.95 CORR
- HCX PL APO 40x/1.10 W CORR
- HC PL APO 100x/1.47 OIL CORR TIRF
Equipment Details:
- Leica DMi8 Inverted Microscope
- Yokagawa CSU-W1 SoRA
- 50um disk
- SoRa disk
- SoRa Mag 1x, 2.8x, 4.0x
- Zt405/488/561/640 dichroic and 100%mirror
- Yokogawa Uniformizer
- Photometrics Kinetix camera
- Photometrics Prime BSI camera
- Mightex Spatial Illuminator
- Microvolution Deconvolution Software
- ASI motorized stage with Piezo top plate (500 um travel)
- OkoLab Cage Incubator
Light Source:
- Vortran Laser Launch
Excitation Lasers:
- 401 nm
- 487 nm
- 561 nm
- 639 nm
Emission Filters:
- Spinning Disk
- Chroma ET 460/50
- Chroma ET 605/70
- Chroma ET 700/75
- Chroma ET 525/50
- Chroma ZET405/488/561/635 m
- Widefield
- DAPI filter cube
- FITC filter cube
- Rhod filter cube
- 405nm LP Dichroic for Mightex
Interface Requirements
Firmware
PC
Windows 10 Pro
Software
- Micro Manager 2.0
