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This is a conventional widefield system for both epifluorescence and brightfield imaging and is equipped with CO2 and a temperature-controlled incubator for live-cell imaging. The fully automated system enables large image acquisition and multi-position high throughput imaging of plates. It is equipped with a Hamamatsu Flash 4.0 camera and a Leica color camera. Leica Thunder software module is also available on this system.

Objectives:

  • N PLAN 5x/0.12 PH0
  • HC PL APO 20x/0.80
  • HC PL APO 40x/0.95 CORR
  • HC PL APO 63x/1.40-0.60 OIL


Equipment Details:

  • Leica DMi8 Inverted Microscope
  • Quantum HS linear motorized stage (Pecan)
  • Hamamatsu Flash 4.0 v3
  • Leica DFC9000 GTC color camera
  • OkoLab Cage Incubator
  • Thunder Software Package


Light Source:

  • LED 8 with Sequencer (395, 438, 475, 511, 555, 575, 635, 730 nm) with 3mm LLG


Emission Filters:

  • 405:
    • EX: 375-435
    • DC: 455
    • EM: 450-490
  • CYR 71010
    • EX: 422-450; 495-517; 566-590; 710-750
    • DC: 459; 523; 598; 763
    • EM: 462-484; 527-551; 602-680; 770-850
  • DFT51010
    • EX: 375-407; 462-496;542-566; 622-654
    • DC: 415; 500; 572; 660
    • EM: 420-450; 506-532; 578-610; 666-724
  • GFP
    • EX: 450-490
    • DC: 495
    • EM: 500-550

Interface Requirements


Firmware

PC

  • Windows Pro

Software

  • LAS-X

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