FPBASE
Optics
Objectives
Plan Fluor 10x/0.3 (Ph1) 654nm/pixel
Plan Apo 20x/0.75 (DIC N2 / 20X) 327nm/pixel
Plan Fluor 40x/0.75 (DIC N2 / 40X I) 164nm/pixel
Plan Fluor 40x/1.3 Oil (DIC N2/ 40X II) 164nm/pixel
Plan Apo 60x/1.2 WI (DIC N2/ 60X II) 109nm/pixel
Plan Apo 100x/1.4 Oil (Ph3) 65.4nm/pixel
Filter Turret
Semrock DAPI/FITC/TRITC/Cy5 Sedat Quad
GFP/mRFP1
CFP/YFP
Analyzer
QLIPP analyzer (Circular Polarizer for 532nm, Right-handed (ThorLabs CP1R532))
FURA2 (Emission Filter within Cube)
Excitation from Lambda 421
| LED wavelength | Filter in front of LED |
|---|---|
| 340 nm | FF01-340/26-25-STR |
| 380 nm | FF01-389/38-25-STR |
| 440 nm | FF01-445/40-25-STR |
| 480 nm | FF01-480/40-25-STR |
| 530 nm | FF01-504/12-STR |
| 561 nm | FF01-562/40-25-STR |
| 630 nm | FF01-630/38-25-STR |
Emission Wheel
Fura2 emission filter
Empty
440/11m Semrock Sedat Quad
525/30m Semrock Sedat Quad
607/36m Semrock Sedat Quad
684/24m Semrock Sedat Quad
ET525/50m GFP/mCherry ET (89021 Em2)
ET632/60m GFP/mCherry ET (89021 Em1)
ET470/24m CFP/YFP (89002 Em2)
ET535/30m CFP/YFP (89002 Em1)
Hardware
Nikon Ti-E Microscope
External Phase Unit
Nikon motorized stage
Sutter Lambda 421 LED light source with NIDAQ interface
Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM7, 128k Baud Rate)
Hamamatsu Flash 4.0 (Right port)
OkoLab incubator and CO2 control
- QLIPP components above condenser
- Semrock Brightline FF01-540/50-25
- ThorLabs LPVISA100, Linear Polarizer, 480-550 nm
- Meadowlark LCs in 3D printed mount
- 1st LC oriented 45 deg relative to the long edge of the housing
- 2nd LC oriented parallel to the long edge of the housing
Interface Requirements
1 serial port for 10-3
3 USB connectors
10-B COM7 (USB)
NI PCI 6723 board
Firmware
Ti: Combination K
TiControl: 5.50
PC
NIC-HighSpeed2
Intel Core i7 4770K (3.50 GHz)
32 GB RAM
Windows 10 Enterprise 64bit
238 GB SSD Hard drive
1 TB SSD Hard drive array (Raid 0)
2 TB Hard drive (RAID 1)
NVIDA GeForce GTX 550Ti
Active Silicon FireBird Card
Software
NIS-Elements 5.21.03 (Build 1489)
Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments)
ET340x Fura-2 (79000 Ex1)
ET380x Fura-2 (79000 Ex2)
387/11x Semrock Sedat Quad
485/20x Semrock Sedat Quad
560/25x Semrock Sedat Quad
650/13x Semrock Sedat Quad
ET470/40x GFP/mCherry ET (89021 Ex2)
ET572/35x GFP/mCherry ET (89021 Ex1)
ET430/24x CFP/YFP (89002 Ex1)
ET500/20x CFP/YFP (89002 Ex2)
