Spinning Disk Confocal

FPBASE

Optics

Objectives

1. Plan Fluor 10x /0.3 (Ph1 DL) -910 nm/pixel

2. Plan Apo 40x/0.95 Corr (DIC N2 / 40X I) - 227.5 nm/pixel

3. Plan Apo VC 60x/1.2 WI- 154nm/pixel

4. Plan Apo VC 60x/1.4 Oil -154 nm/pixel

5. Plan Apo VC 100x/1.4 Oil (DIC N2 / 100X I) 91 nm/pixel

6. Apo TIRF 100x/1.49 Oil 91 nm/pixel

Filter Turret 1 (FL1, Top)

1. Empty

2. DIC Analyzer

3. GFP (long pass)

4. YFP

5. Rhodamine

6. D/F/R

Yokogawa Dichroics

1. 405/491/561/640

Emission Wheel

(To get NIS-Elements numbering, add 1 to these numbers)

1. Open

2. ET460/50m

3. ET525/50m

4. ET645/65m

5. ET700/75m

6. ET610/60m

7. zET405/488/561/635m

8. Empty

9. Empty

10. Empty

Hardware

• Nikon Ti-E Microscope

• Yokogawa CSU-22

• Sutter Lambda LS-2 lamp for epifluorescence illumination

• Sutter Emission wheel controlled by Lambda 10-B

• Sutter TLED brightfield illumination

• Photometrics Evolve Delta

• Prior motorized stage with Piezo Z-drive

• Okolab enclosure and Bold Line incubator

Lasers

• 405 nm, 100 mW Coherent OBIS

• 488 nm, 150 mW Coherent OBIS

• 561 nm, 100 mW Coherent OBIS

• 640 nm, 100 mW Coherent OBIS

Interface Requirements

• 1 serial port for Sutter Lambda 10-B (COM5) 

• 1 serial port for Yokogawa CSU-22 (COM)

• 1 PCIe to highspeed serial port for Prior Proscan II (COM6)

• Firewire for Evolve

• USB for Ti

• USB for laser controller (COM4; MM only)

Firmware

• Ti: Combination K

• Ti-El: V5.2 Sum Fa24h

• TiPad: V5.1 Sum 08C4h
• TiHUBC/A Hub Controller A: V5.2 Sum C183h, V5.2 Sum 340Bh

PC

• Windows 10 Enterprise

• Core i7 3.4GHz

• 16 GB RAM

• NVidia GTX550Ti

• 240GB SSD

• 2TB Hard drive (RAID 1)

Software

• Nikon Elements 5.02 build 1266

• Micro-Manager 2.0 gamma (20210310)- Java 8.0282.8

• Micro-Manager 1.4 (20170102)