N-SIM

FPBASE

Optics

Objectives

1. Plan Apo I 10x/0.45

2. Plan Fluor ELWD 40x/0.65

3. Empty

4. Empty

5. Plan Apo IR 60x/1.27 Water

6. Apo TIRF 100x/1.49 Oil

Filter Turret 1

1. Empty

2. Empty

3. Analyzer

4. UV-2A

5. FITC

6. HYQ TRITC

Filter Turret 2

1. 488

2. 405

3. 561

4. Empty

5. H/M

6. 647

SIM Gratings

• 3D EX V-G 100X/1.49 (for 100x objective, 405nm - 561nm)

• 3D EX V-R 100x/1.49 (for 100x objective, 405nm - 640nm)

• 3D EX V-R 60x/1.27 - TIRF 488 100x/1.49 (for 60x objective, 405nm - 640nm or 100x objective, 488nm TIRF)

• TIRF561 100x/1.49

• 3D 1layer 100x/1.49

• 3D 1layer 60x/1.27 - TIRF488 100x/1.49

Hardware

• Nikon Ti-E Microscope

• Nikon motorized stage with piezo Z insert

• Andor DU897 on right port

• Nikon LUSU laser controller

• Nikon Intensilight

Interface Requirements

• USB for MCL nano drive
• USB for Nikon TI
• USB For Nikon AOTF
• Camera Card for DU897

Firmware

• Ti-E: v4.01

• HUB-AM: v3.20

• HUB-AS: v4.00

• Remote: v1.25

• Ti-JOY: v1.20

PC

• HP Z400

• Xeon W3670 (3.2GHz)

• 24 GB RAM

• 64 bit Windows 7

• 2 TB Hard drive

• NVidia Quadro Pro 600

Software

• NIS-Elements 5.02.00 (build 1266) Patch 02