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Huygens is an easy-to-use commercial deconvolution program. We have it installed on Deconvolution Workstation 3 and older version are on the other two Deconvolution Workstations.

Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective3.


If you are doing deconvolution on images acquired on the Spinning Disk here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification for the CSU-22.

MagnificationRadiusSpacing Distance
100X250nm2.53um
60X416.7nm4.22um
40X625nm6.22um
20X1250nm12.7um
10X2500nm12.7um


Here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification for the CSU-W1.

MagnificationRadiusSpacing Distance
100X250nm5um
60X416.7nm8.33um
40X625nm15um
20X1250nm30um
10X2500nm50um
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