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Huygens is an easy-to-use commercial deconvolution program. We have it installed on Deconvolution Workstation 3 and older version are on the other two Deconvolution Workstations.

Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective4.


Pinhole Calculator link for the Nikon TE2000-E with C2 scanning head


CSU-22 Back projected Pinhole radius and spacing distance  by magnification 

MagnificationRadiusSpacing Distance
100X250nm2.53um
60X416.7nm4.22um
40X625nm6.22um
20X1250nm12.7um
10X2500nm12.7um


CSU-W1 Back projected Pinhole radius and spacing distance  by magnification 

MagnificationRadiusSpacing Distance
100X250nm5um
60X416.7nm8.33um
40X625nm15um
20X1250nm30um
10X2500nm50um

CREST-V2 Back projected Pinhole radius and spacing distance  by magnification 

MagnificationRadiusSpacing Distance
100X250nm2.5um
60X416.7nm4.16um
40X625nm6.25um
20X1250nm12.5um
10X2500nm25um
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