Huygens is an easy-to-use commercial deconvolution program. We have it installed on Deconvolution Workstation 3 and older version are on the other two Deconvolution Workstations.
Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective4.
CSU-22 Back projected Pinhole radius and spacing distance by magnification
| Magnification | Radius | Spacing Distance |
|---|
| 100X | 250nm | 2.53um |
| 60X | 416.7nm | 4.22um |
| 40X | 625nm | 6.22um |
| 20X | 1250nm | 12.7um |
| 10X | 2500nm | 12.7um |
CSU-W1 Back projected Pinhole radius and spacing distance by magnification
| Magnification | Radius | Spacing Distance |
|---|
| 100X | 250nm | 5um |
| 60X | 416.7nm | 8.33um |
| 40X | 625nm | 15um |
| 20X | 1250nm | 30um |
| 10X | 2500nm | 50um |
CREST-V2 Back projected Pinhole radius and spacing distance by magnification
| Magnification | Radius | Spacing Distance |
|---|
| 100X | 250nm | 2.5um |
| 60X | 416.7nm | 4.16um |
| 40X | 625nm | 6.25um |
| 20X | 1250nm | 12.5um |
| 10X | 2500nm | 25um |
