Huygens is an easy-to-use commercial deconvolution program. We have it installed on Deconvolution Workstation 3 and older version are on the other two Deconvolution Workstations.
Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective3.
If you are doing deconvolution on images acquired on the Spinning Disk here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification for the CSU-22.
| Magnification | Radius | Spacing Distance |
|---|---|---|
| 100X | 250nm | 2.53um |
| 60X | 416.7nm | 4.22um |
| 40X | 625nm | 6.22um |
| 20X | 1250nm | 12.7um |
| 10X | 2500nm | 12.7um |
Here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification for the CSU-W1.
| Magnification | Radius | Spacing Distance |
|---|---|---|
| 100X | 250nm | 5um |
| 60X | 416.7nm | 8.33um |
| 40X | 625nm | 15um |
| 20X | 1250nm | 30um |
| 10X | 2500nm | 50um |
