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| Protein | λex | λem | Contrast Ratio | Nphotons (median) | Notes | Reference |
|---|---|---|---|---|---|---|
| PS-CFP2 | 490 | 511 | 2000 | 260 | Cyan-to-green photoswitchable | 6, Evrogen |
| mGeos-M | 503 | 514 | 387 | Reversibly photoswitchable | 7 | |
| PATagRFP | 562 | 596 | 540 | 500? | Photoactivatible | 8, Evrogen |
| PAmCherry | 564 | 595 | 4000 | 348 | Photoactivatible | 1 |
| tdEos | 569 | 581 | 499 | Green-to-red photoswitchable | 3,4,5 | |
| mEos2 | 573 | 584 | 379 | Green-to-red photoswitchable | 3,4 | |
| mEos3.2 | 572 | 580 | 482 | Green-to-red photoswitchable | 3 | |
| PSmOrange | 631 | 662 | 10700 | 337 | Orange-to-far red photoswitchable | 2 |
1. Subach, FV. et al., Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nat. Meth. 2009, 6: p. 153-159.
2. Subach, OM. et al., A photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat. Meth. 2011, 8: p. 771-777.
3. Zhang, M. et al., Rational design of true monomeric and bright photoactivatable fluorescent proteins. Nat. Meth. 2012, 9: p. 727-729.
4. McKinney, SA. et al., A bright and photostable photoconvertible fluorescent protein. Nat Meth. 2009, 6: p. 131-133.
5. Wiedenmann, J. et al., EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc Natl Acad Sci 2004. 101(45): p.15905-15910.
6. Shroff, H. et al., Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13.
7. Chang, H. et al., A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4455-60.
8. Subach, FV. et al., Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. J Am Chem Soc. 2010 May 12;132(18):6481-91.
