CVRI Nikon Epifluorescence Microscope

FPBASE

Optics

Objectives

• Plan Apo 4x/0.2
• Plan Fluor 10x/0.3
• Plan Apo VC 0.75NA 20x
• Plan Apo 20X/0.45 ELWD
• Plan Apo VC 60x/1.4

Filter Turret 

• DAPI
• FITC 
• TRITC
• Cy5 
• mCherry-LP 

Hardware

• Nikon Ti  Inverted Microscope
• Andor Clara Interline CCD (USB, Left port) 
• In Vivo Scientific Cage incubator
• Oko CO2 Mixer
• Sutter Lambda XL lamp (COM 4, Baud rate Auto)
• ASI MS2000 XY stage (COM3)

PC

• Windows 10 64-bit

• Xeon W3505, 2.3 GHz

• 6 GB RAM

• ATI FirePro 2260

• 500GB SSD

Firmware

• Ti-E V.5.1

Software

• Nikon Elements 5.02 build 1266

• Micro-Manager 2.0

• Micro-Manager 1.4