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General Microscopy

Michael Davidson maintains an excellent family of websites, many sponsored by manufacturers, which are an excellent source of microscopy information:

Fluorescent Probes

  • The Molecular Probes Handbook - a good source of information on fluorescent dyes and small molecule reporters.

  • openoptogenetics - Optogenetics wiki site with good technical information

  • FPbase- a good source of information on fluorescent proteins. Spectra viewer also useful for comparison of filter sets between different fluorescent proteins.

Data Analysis



  • Image.sc - Community forum for multiple open-source image analysis software packages where you can find answer to common problems and post questions to the community. The forum is very active and you can get help quickly.

  • Microforum- Community forum based on hardware, acquisition, and specimen-related question.


Recommended Books on Microscopy

Fundamentals of Light Microscopy and Electronic Imaging, Douglas B. Murphy: A good general purpose introduction to commonly used microscopy techniques, including fluorescence, phase contrast and DIC, and brief discussions of confocal microscopy and cameras.

Handbook of Biological Confocal Microscopy, 3rd ed., James Pawley, ed.: This is the book to get if you want an in-depth understanding of not just confocal microscopy, but the whole diversity of modern microscopy techniques. It's not a good introduction to the subject but it is a fantastic reference work with lots and lots of technical detail.

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