FPBASE
Notes on Usage
Make sure your sample is centered in the cuvette. Imaging close to the edges of the cuvette often induces aberrations that result in poor image quality.
In general, the 5mm slit provides the best balance of light sheet width and taper. The smaller slits result in thicker light sheets with little benefit, unless you are using the 1x objective with a low zoom.
Don't use zooms less than 2x unless absolutely necessary. There are substantial aberrations at the edges of field of view at low zooms.
At 3x zoom and below, set the CameraCrop parameter to 2048 x 2048 - this eliminates aberrations present at the edges of the field of view at these lower magnifications.
With the 5x objective, you may need to adjust the AZ100 Z position in steps as small as 2 um to get an accurate focus.
Optics
Objectives
AZ Plan Fluor 2x
- AZ Plan Fluor 5x
- AZ Plan Apo 1x
Emission Wheel
Position 0: Open
Position 1: ET455/50m
Position 2: ET535/36m
Position 3: ET605/52m
Position 4: ET705/72m
Position 5: Open
Position 6: Closed
Position 7: Closed
Turret (Manual for EPI)
- Empty
- 96362 ET GFP C94925
- 96365 ET TXR C93169
- 96345 HQ:Y GFP C77392 (from C1)
Hardware
Nikon AZ100
- Vortran 4-line Laser Launch
Andor Zyla 5.5 Camera
- Custom light sheet optics
Lasers
- 405nm 100mW
- 488nm 50mW
- 561nm 50mW
- 640nm 110mW
Interface
- 1 USB Arduino-Hub (COM5, baud Rate 57600)
- ASI MFC-2000 Z stage (COM1, baud rate 9600)
- 1 Camera card slot-Zyla 5.5
- 1 serial port ASI Controller, XYZ Stage, Camera stage (COM3 baud rate 115200)
- COM2 baud rate 115200
- 1 USB for Vortan
- 1 USB for Az100
PC
Core i7 4770K (4 Ghz)
32 GB RAM
NVidia GTX970
Windows 10 Enterprise 64bit
250GB SSD
- 2TB Data drive
