6D

FPBASE

Optics

Objectives

1. Plan Apo 4x/0.2

2. Plan Apo 10x/0.45

3. Plan Apo 20x/0.75

4. Plan Apo 40x/0.95 Corr

5. Plan Apo VC 60x/1.4 Oil

6. Plan Apo VC 100x/1.4 Oil (DIC N2 / 100X I) 65.4nm/pixel

(other objectives)

• Plan Apo 2x/0.1

• Plan Fluor 10x/0.3

• Super Fluor 40x/1.3 oil

• Super Fluor 100x/0.5-1.3 iris

Filter Turret

1. Analyzer

2. Empty

3. Empty

4. CFP/YFP (89002)

5. GFP/mCherry ET (89021)

6. Semrock 5-band FF408/504/581/667/762 (DA/FI/TR/Cy5/Cy7-5X5M-B-000)

Excitation Wheel

1. 387/11x Semrock 5-band

2. 485/20x Semrock 5-band

3. 560/25x Semrock 5-band

4. 650/13x Semrock 5-band

5. 740/13x Semrock 5-band

6. ET470/40x GFP/mCherry ET (89021 Ex2)

7. ET572/35x GFP/mCherry ET (89021 Ex1)

8. ET430/24x CFP/YFP (89002 Ex1)

9. ET500/20x CFP/YFP (89002 Ex2)

10. Open

Emission Wheel

1. Open

2. 440/40m Semrock 5-band

3. 525/30m Semrock 5-band

4. 607/36m Semrock 5-band

5. 684/24m Semrock 5-band

6. 809/81m Semrock 5-band

7. ET525/50m GFP/mCherry ET (89021 Em2)

8. ET632/60m GFP/mCherry ET (89021 Em1)

9. ET470/24m CFP/YFP (89002 Em2)

10. ET535/30m CFP/YFP (89000 Em1)

Hardware

• Nikon Ti-E Microscope

• Sutter Lambda LS lamp with filter wheel and shutter

• Sutter Lambda 10-3 (COM 4)

• Sutter Emission wheel and brightfield shutter

• Nikon DS-Qi2 (fluorescence)

• Nikon DS-Ri2 (color)

Interface Requirements

•  1 x USB for Nikon TI-E
• 1x USB for DS-Qi2 Camera
• 1 X USB DS-Ri2 Camera
• 1x USB Sutter Lambda 10-3

Firmware

TI-E: v5.2

PC

• Core i7 4770K (3.5 Ghz)

• 32 GB RAM

• NVidia GTX760

• Windows 10 Enterprise 64bit 

• 256 GB SSD
• 2TB HD (RAID 1)

Software

• NIS-Elements 5.21.03 (b1489_64bit)